In Vitro Cytotoxic and Anti-inflammatory Activities of Tanacetum argenteum (Lam.) Willd. subsp. argenteum Extract
The objective of this study was to examine the anti-inflammatory and cytotoxic potential of n-hexane, ethyl acetate, and methanolic extracts of subsp. . L. is the third largest genus of family and is represented by 60 taxa in Turkish flora. Sesquiterpene lactones and pyrethrins are the main chemical...
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| Published in | Turkish journal of pharmaceutical sciences Vol. 14; no. 3; pp. 231 - 236 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Turkey
Türk Eczacılar Birliği
01.12.2017
Galenos Publishing |
| Subjects | |
| Online Access | Get full text |
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| Abstract | The objective of this study was to examine the anti-inflammatory and cytotoxic potential of n-hexane, ethyl acetate, and methanolic extracts of
subsp.
.
L. is the third largest genus of
family and is represented by 60 taxa in Turkish flora. Sesquiterpene lactones and pyrethrins are the main chemical groups of the genus.
subsp.
is an endemic taxa that is distributed in the Central and South Anatolia.
anti-inflammatory activity was assayed using iNOS and NF-κB inhibition tests on RAW264.7 and HeLa cells. The cytotoxic activities were tested against ten cell lines using MTT assays.
As a result, the n-hexane extract was found more active than the positive control parthenolide in iNOS test (IC
: 0.627±0.16 μg/mL) and cytotoxic experiments against PC3 and MPANC-96 cell lines (IC
: 2.85±0.51 μg/mL and 5.35±1.24 μg/mL, respectively). |
|---|---|
| AbstractList | Objectives: The objective of this study was to examine the anti-inflammatory and cytotoxic potential of n-hexane, ethyl acetate, and methanolic
extracts of Tanacetum argenteum subsp. argenteum.
Materials and Methods: Tanacetum L. is the third largest genus of Asteraceae family and is represented by 60 taxa in Turkish flora. Sesquiterpene
lactones and pyrethrins are the main chemical groups of the genus. T. argenteum subsp. argenteum is an endemic taxa that is distributed in the
Central and South Anatolia.
Results: In vitro anti-inflammatory activity was assayed using iNOS and NF-κB inhibition tests on RAW264.7 and HeLa cells. The cytotoxic activities
were tested against ten cell lines using MTT assays.
Conclusion: As a result, the n-hexane extract was found more active than the positive control parthenolide in iNOS test (IC50: 0.627±0.16 μg/mL) and
cytotoxic experiments against PC3 and MPANC-96 cell lines (IC50: 2.85±0.51 μg/mL and 5.35±1.24 μg/mL, respectively). OBJECTIVESThe objective of this study was to examine the anti-inflammatory and cytotoxic potential of n-hexane, ethyl acetate, and methanolic extracts of Tanacetum argenteum subsp. argenteum. MATERIALS AND METHODSTanacetum L. is the third largest genus of Asteraceae family and is represented by 60 taxa in Turkish flora. Sesquiterpene lactones and pyrethrins are the main chemical groups of the genus. T. argenteum subsp. argenteum is an endemic taxa that is distributed in the Central and South Anatolia. RESULTSIn vitro anti-inflammatory activity was assayed using iNOS and NF-κB inhibition tests on RAW264.7 and HeLa cells. The cytotoxic activities were tested against ten cell lines using MTT assays. CONCLUSIONAs a result, the n-hexane extract was found more active than the positive control parthenolide in iNOS test (IC50: 0.627±0.16 μg/mL) and cytotoxic experiments against PC3 and MPANC-96 cell lines (IC50: 2.85±0.51 μg/mL and 5.35±1.24 μg/mL, respectively). The objective of this study was to examine the anti-inflammatory and cytotoxic potential of n-hexane, ethyl acetate, and methanolic extracts of subsp. . L. is the third largest genus of family and is represented by 60 taxa in Turkish flora. Sesquiterpene lactones and pyrethrins are the main chemical groups of the genus. subsp. is an endemic taxa that is distributed in the Central and South Anatolia. anti-inflammatory activity was assayed using iNOS and NF-κB inhibition tests on RAW264.7 and HeLa cells. The cytotoxic activities were tested against ten cell lines using MTT assays. As a result, the n-hexane extract was found more active than the positive control parthenolide in iNOS test (IC : 0.627±0.16 μg/mL) and cytotoxic experiments against PC3 and MPANC-96 cell lines (IC : 2.85±0.51 μg/mL and 5.35±1.24 μg/mL, respectively). |
| Abstract_FL | Amaç: Tanacetum argenteum subsp. argenteum bitkisinin hekzan, etil asetat ve metanol ekstrelerinin anti-inflamatuvar ve sitotoksik potansiyellerini
incelemektir.
Gereç ve Yöntemler: Tanacetum L. Asteraceae familyasının en büyük 3. cinsidir ve Türkiye florasında 60 taksonla temsil edilir. Seskiterpen laktonlar
ve piretrinler cinsin ana kimyasal gruplarıdır. T. argenteum subsp. argenteum İç ve Güney Anadolu’da yayılış gösteren endemik bir taksondur.
Bulgular: In vitro anti-inflamatuvar aktivite iNOS ve NF-κB inhibisyon metodlarıyla RAW264.7 ve HeLa hücrelerine karşı test edilmiştir. Sitotoksik
aktivite MTT metoduyla 10 hücre hattına karşı denenmiştir.
Sonuç: Sonuç olarak, n hekzan ekstresi iNOS testinde (IC50: 0.627±0.16 μg/mL) ve PC3 ve MPANC-96 hücreleri (IC50: 2.85±0.51 μg/mL ve 5.35±1.24
μg/mL, sırasıyla) üzerinde yapılan sitotoksik ölçümlerde pozitif kontrol partenolitten daha etkili bulunmuştur. |
| Author | Baykan, Şüra Albayrak, Gökay Nalbantsoy, Ayşe |
| AuthorAffiliation | Eczacılık Meslek Bilimleri Bölümü, Farmasotik Botanik Anabilim Dalı Biyomühendislik Bölümü, Biyomühendislik Anabilim Dalı Temel Eczacılık Bilimleri Bölümü, Farmasotik Botanik Anabilim Dalı |
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| DocumentTitleAlternate | Tanacetum argenteum subsp. argenteum ekstrelerinin in vitro sitotoksik ve anti-inflamatuvar etkileri |
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| Keywords | anti-inflammatory NF-κB iNOS Tanacetum argenteum subsp. argenteum MTT cytotoxicity sitotoksisite anti-inflamatuvar |
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.
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| Title | In Vitro Cytotoxic and Anti-inflammatory Activities of Tanacetum argenteum (Lam.) Willd. subsp. argenteum Extract |
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