Enamel neonatal line thickness in deciduous teeth of Australian children from known maternal health and pregnancy conditions

• Published in: Early human development Vol. 137; p. 104821
• Main Authors: Behie, Alison M., Miszkiewicz, Justyna J.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.10.2019
• Subjects: Adolescent; Adult; Alcohol; Alcohol Drinking - epidemiology; Australia; Child; Dental Enamel - anatomy & histology; Dental Enamel - growth & development; Dental histology; Enamel; Female; Humans; Infant, Newborn; Male; Maternal Health; Neonatal line; Perinatal; Postnatal; Pregnancy; Prenatal; Prenatal Exposure Delayed Effects - epidemiology; Tooth, Deciduous - anatomy & histology; Tooth, Deciduous - growth & development; Australia; Prenatal; Postnatal; Enamel; Perinatal; Alcohol; Dental histology; Neonatal line
• ISSN: 0378-3782; 1872-6232
• DOI: 10.1016/j.earlhumdev.2019.07.004

Physiological disruptions to early human development have implications for health and disease in later life. Limited research has explored how prenatal factors influence dental development in children of mothers with known pregnancy conditions. Enamel in human deciduous teeth begins forming in utero and is highly susceptible to physiological upsets experienced perinatally. The moment of birth itself is marked in deciduous enamel by the Neonatal Line (NNL) as a baby transitions from the uterine to external environment. This study evaluates the effect of maternal health factors that include stress and alcohol consumption on NNL in teeth from Australian children. Mothers (n = 53) were interviewed about their health during pregnancy and experience of birth. Sixty-five deciduous teeth (incisors, molars, one canine) from their children were donated for histological examination. Neonatal line thickness was measured from thin sections and evaluated against maternal and neonatal factors using statistical analyses, controlling for tooth type and birth number. The only maternal factor of a statistically significant effect on NNL thickness was alcohol consumption. Children of mothers who drank occasionally during pregnancy had a thicker NNL when compared to children of mothers who abstained. These results suggest that maternal lifestyle factors influence NNL formation possibly due to physiological changes that disrupt calcium homeostasis during enamel deposition. We highlight large intra-specific variation in human NNL expression. The potential of dental sampling in identifying children with prenatal exposure to alcohol is suggested. •A microscopic deciduous tooth “birth marker” is known as the Neonatal Line (NNL).•NNL can be used to study a baby's pre- and perinatal environment.•Results show prenatal exposure to alcohol has the strongest effect on NNL.•Other maternal health factors are not linked to NNL in our sample.•We highlight a large extent of intra-specific variation in NNL expression.