Preparation of medium and long chain triacylglycerols by lipase-catalyzed interesterification in a solvent-free system

• Published in: Process biochemistry (1991) Vol. 54; pp. 89 - 95
• Main Authors: Lu, Jiyuan, Jin, Qingzhe, Wang, Xingguo, Wang, Xiaosan
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.03.2017; Elsevier BV
• Subjects: Acids; Biocatalyst; Chains; Chemical synthesis; Chinese national standard; Diglycerides; Glycerol; Heating; Interesterification; Lipase; Medium and long chain triacylglycerols; Medium chain fatty acids; Neutralization; Oils & fats; Peroxide; Pharmaceutical industry; Purification; Reaction time; Silica; Silica gel; Silicon dioxide; Solvents; Soybean oil; Triglycerides; Medium and long chain triacylglycerols; Interesterification; Medium chain fatty acids; Chinese national standard; Biocatalyst
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2016.12.015

[Display omitted] •Lipozyme 435-catalyzed interesterification is applied to prepare MLCTs.•A two-step purification is used to reduce polar compounds to an acceptable level.•The quality of purified product is found to meet the standard JX20070100. Synthesis of medium and long chain triacylglycerols (MLCTs) by lipase-catalyzed interesterification of soybean oil with medium chain triacylglycerols (MCTs) in a solvent-free system is investigated in this study. Firstly, reaction conditions of the interesterification were optimized. Under the optimum conditions (Lipozyme 435 of 8% as catalyst, substrate ratio of 40:60, heating temperature of 90°C, reaction time of 6h), MLCTs were obtained in a 74.9% yield Secondly, a two-step purification was conducted to reduce acid, peroxide values and diacylglycerol content to acceptable levels. After the deacidification by the neutralization and silica gel absorption, acid, peroxide values and diacylglycerol content were reduced to 0.14mg/g, 1.5mmol/kg and 1.2%, respectively. The determined levels of physicochemical parameters of purified MLCTs fell within the permitted ranges of the Chinese national standard specification for MLCTs. After a two-step purification, a qualified product was produced and can be used in pharmaceutical industry as a component of fat emulsion injection. The process for the preparation of MLCTs is scalable. Differently from previous studies, the aim of this study is to obtain MLCTs products meeting the national standard JX20070100 for structured triacylglycerols.