Unraveling rapid extraction of fucoxanthin from Padina tetrastromatica: Purification, characterization and biomedical application

• Published in: Process biochemistry (1991) Vol. 73; pp. 211 - 219
• Main Authors: Raguraman, Vasantharaja, L., Stanley Abraham, D., MubarakAli, G., Narendrakumar, R., Thirugnanasambandam, R., Kirubagaran, N., Thajuddin
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.10.2018; Elsevier BV
• Subjects: Adenosine; Algae; Antioxidants; Biochemistry; Biocompatibility; Biomedical materials; Biomolecule; Biomolecules; Carotenoids; Cytotoxicity; Free radicals; Fucoxanthin; Functional foods & nutraceuticals; High-performance liquid chromatography; HR-MS; Hydrogen peroxide; Liquid chromatography; Phenolic compounds; Phenols; Purification; Response surface methodology; Retention time; Toxicity; Ultrasound; Ultrasound-assisted extraction; Water purification; Biomolecule; Antioxidants; Fucoxanthin; HR-MS; Ultrasound-assisted extraction
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2018.08.006

[Display omitted] •Ultrasound assisted extraction was adopted for the extraction of fucoxanthin.•Parameters were optimized using RSM for rapid extraction and maximum yield.•Extracted fucoxanthin showed pure as same as that of standard.•Extracted fucoxanthin exhibited antioxidant and cytoprotective effects. Fucoxanthin, a group of carotenoids abundantly present in the marine macro algae which exhibits diverse health benefits. Extraction of such potential biomolecules involves multiple steps in conventional extraction (CE) and also laborious and time-consuming. We attempted to extract high percentage fucoxanthin and phenolic compounds from macro alga, Padina tetrastromatica adopting ultrasound-assisted extraction (UAE). It was further optimized with solvent concentration; temperature and time as 80%, 50 °C and 30 min respectively using one factor at a time method (OFAT) followed by response surface methodology (RSM). It was observed that fucoxanthin yield of about 750 μg g−1 DW was obtained at the maximum by UAE than that of CE (430 μg g-1 DW). It was further purified and characterized using FTIR, HPLC and HRMS technique confirming the presence of purified fucoxanthin at the retention time of 3.14 min and molecular mass of 681.41 m/z. In addition, fucoxanthin exhibits a strong antioxidant activity by inhibiting DPPH and ABTS free radicals upon dose dependent manner. Interestingly, fucoxanthin showed protective effect against H2O2-induced intracellular ROS and cytotoxicity on fibroblast cells at the concentration of 120 μM. Thusly, UAE could be implemented for the extraction at high percentage and eventually be utilized these biomolecules as nutraceuticals.