Effects of cysteine protease inhibitors on rabbit cathepsin D maturation

• Published in: The American journal of physiology Vol. 257; no. 6 Pt 1; p. C1069
• Main Authors: Samarel, A M, Ferguson, A G, Decker, R S, Lesch, M
• Format: Journal Article
• Language: English
• Published: United States 01.12.1989
• Subjects: Animals; Cathepsin D - biosynthesis; Cathepsin D - genetics; Cells, Cultured; Chloroquine - pharmacology; Cysteine Proteinase Inhibitors - pharmacology; Fibroblasts - drug effects; Fibroblasts - enzymology; Kinetics; Leucine - analogs & derivatives; Leucine - pharmacology; Leupeptins - pharmacology; Methionine - metabolism; Myocardium - enzymology; Protein Processing, Post-Translational - drug effects; Rabbits; Sulfur Radioisotopes

To examine the effects of cysteine protease inhibitors on cathepsin D intracellular transport, proteolytic processing, and secretion, primary cultures of rabbit cardiac fibroblasts were grown to confluence and exposed (24 h) to media containing leupeptin (0-10 mM), E 64 (0-10 mM), or chloroquine (0-...