Preliminary studies on the antibacterial activity of crude extracts and alkaloids from species of Aspidosperma

Screening tests of hydroethanolic crude extracts of six species of Aspidosperma (Apocynaceae) against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa were performed. Aspidosperma ramiflorum Muell. Arg. showed good activity against Bacillus subtilis with MIC and...

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Published inPharmaceutical biology Vol. 47; no. 11; pp. 1085 - 1089
Main Authors Oliveira, Arildo Jose Braz de, Koike, Luzia, Reis, Francisco Assis Machado de, Eguchi, Silvia Yuko, Endo, Eliana Harue, Nakamura, Celso Vataru, Dias Filho, Benedito Prado
Format Journal Article
LanguageEnglish
Published Informa UK Ltd 01.11.2009
Taylor & Francis
Subjects
Alkaloids
antibacterial activity
antibacterial properties
Aspidosperma
bark
bark extracts
fractionation
medicinal plants
minimum bactericidal concentration
minimum inhibitory concentration
plant extracts
stem bark
time-kill
traditional medicine
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Summary:Screening tests of hydroethanolic crude extracts of six species of Aspidosperma (Apocynaceae) against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa were performed. Aspidosperma ramiflorum Muell. Arg. showed good activity against Bacillus subtilis with MIC and MBC of 15.7 and 125 μg/mL, moderate activity against Staphylococcus aureus with MIC and MBC of 250 and 500 μg/mL, and weak activity against Escherichia coli with MIC and MBC of 1000 μg/mL. Aspidosperma pyricolum Muell. Arg. (MIC/MBC 125/250 μg/mL) and Aspidosperma olivaceum Muell. Arg. (MIC/MBC 250/ > 1000 μg/mL) displayed moderate antibacterial activity against Bacillus subtilis. Separation of the crude extract of Aspidosperma ramiflorum was performed according to the usual acid-base process, which produces alkaloid mixtures and closely related metabolites. The basic fraction was active against Bacillus subtilis, Staphylococcus aureus, and Escherichia coli, with MICs of 31.2, 62.5, and 250 μg/mL, respectively. The basic fractions were more active than the acid fractions, probably because they contained some active alkaloids and/or closely related metabolites absent from the other fractions, or they contained a higher concentration of these active compounds.
ISSN:1388-0209
1744-5116
DOI:10.3109/13880200903008633