Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions

• Published in: Biotechnology advances Vol. 72; p. 108338
• Main Authors: Yin, Chengmei, Chen, Xiangyao, Zhang, Haiyang, Xue, Yong, Dong, Hao, Mao, Xiangzhao
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.05.2024
• Subjects: Cascade; Characterization; Continuous flow; Immobilized enzyme; Interface catalysis; Lipase; Material design; Pickering emulsion; Reactor design; Stimuli-responsive; Characterization; Pickering emulsion; Interface catalysis; Material design; Stimuli-responsive; Reactor design; Lipase; Immobilized enzyme; Cascade; Continuous flow
• ISSN: 0734-9750; 1873-1899
• DOI: 10.1016/j.biotechadv.2024.108338

Non-homogeneous enzyme-catalyzed systems are more widely used than homogeneous systems. Distinguished from the conventional biphasic approach, Pickering emulsion stabilized by ultrafine solid particles opens up an innovative platform for biocatalysis. Their vast specific surface area significantly enhances enzyme-substrate interactions, dramatically increasing catalytic efficiency. This review comprehensively explores various aspects of Pickering emulsion biocatalysis, provides insights into the multiple types and mechanisms of its catalysis, and offers strategies for material design, enzyme immobilization, emulsion formation control, and reactor design. Characterization methods are summarized for the determination of drop size, emulsion type, interface morphology, and emulsion potential. Furthermore, recent reports on the design of stimuli-responsive reaction systems are reviewed, enabling the simple control of demulsification. Moreover, the review explores applications of Pickering emulsion in single-step, cascade, and continuous flow reactions and outlines the challenges and future directions for the field. Overall, we provide a review focusing on Pickering emulsions catalysis, which can draw the attention of researchers in the field of catalytic system design, further empowering next-generation bioprocessing. •Pickering emulsion biocatalysis is focused and systematically reviewed.•Design principles for Pickering emulsion systems are introduced in different design stages.•Different stimuli-responsive reaction systems used for demulsification are reviewed.•Detailed methods for Pickering emulsion characterization are summarized.•Applications and promising prospects of Pickering emulsion biocatalysis are proposed.