Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29

• Published in: Process biochemistry (1991) Vol. 86; pp. 9 - 15
• Main Authors: Schmid, Maximilian T., Song, Hyunjeong, Raschbauer, Michaela, Emerstorfer, Florian, Omann, Markus, Stelzer, Franz, Neureiter, Markus
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.11.2019; Elsevier BV
• Subjects: Bacillus megaterium; Cultivation; Desugarized sugar beet molasses; Fractionation; Halophilic fermentation; Molasses; Poly(3-hydroxybutyrate); Substrates; Sugar industry; Sugar industry by-product; Syrups & sweeteners; Poly(3-hydroxybutyrate); Halophilic fermentation; Desugarized sugar beet molasses; Sugar industry by-product; Bacillus megaterium
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.08.001

[Display omitted] •Cost effective production of poly(3-hydroxybutyrate) (P(3HB)).•Bacillus megaterium uyuni S29 produces 60 g P(3HB) per 100 g cell dry mass.•Desugarized beet molasses is an inexpensive substrate for P(3HB) production.•Simple and robust process for (P(3HB)) production.•P(3HB) polymer consisting of two fractions with a molecular mass of 722 and 189 kDa. Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L−1 with a P(3HB) content of 0.6 g g−1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L−1 h−1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.