Applications of ELISA, Western blotting and immunoaffinity concentration for survey of ginsenosides in crude drugs of Panax species and traditional Chinese herbal medicines

• Published in: Analyst (London) Vol. 125; no. 8; pp. 1425 - 1429
• Main Authors: FUKUDA, N, TANAKA, H, SHOYAMA, Y
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.01.2000
• Subjects: Biological and medical sciences; Blotting, Western; Chromatography, Affinity; Drugs, Chinese Herbal - chemistry; Enzyme-Linked Immunosorbent Assay; General pharmacology; Medical sciences; Panax - chemistry; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plants, Medicinal; Immunoaffinity chromatography; Pharmacognosy; Panax ginseng; Medicinal plant; Chinese medicine; Analysis method; Folk medicine; Dicotyledones; Angiospermae; Immunoblotting assay; Spermatophyta; Qualitative analysis; ELISA assay; Araliaceae; Quantitative analysis
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/b002932g

A combination of ELISA, Western blotting and immunoaffinity concentration using an anti-ginsenoside Rb1 monoclonal antibody was applied for qualitative and quantitative surveys of ginsenoside Rb1 and related ginsenosides in roots and traditional Chinese herbal medicines. To improve the low correlation between ELISA and HPLC analysis, the crude extract of roots was immunoaffinity concentrated to make evident the effect of malonyl ginsenoside Rb1. Immunoaffinity column chromatography also concentrated an unknown ginsenoside which had the same cross-reaction with ginsenoside Rb1.