Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata

White spot syndrome virus (WSSV), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. In this study, benthic larvae of the mud crab Scylla serrata were captured from Taiwan's coastal waters and screened for the prese...

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Bibliographic Details
Published inDiseases of aquatic organisms Vol. 40; no. 2; pp. 157 - 161
Main Authors CHEN, L.-L, LO, C.-F, CHIU, Y.-L, CHANG, C.-F, KOU, G.-H
Format Journal Article
LanguageEnglish
Published Oldendorf Inter-Research 14.03.2000
Subjects
Animal aquaculture
Animal productions
Animals
Biological and medical sciences
Brachyura - virology
Crustacea
DNA Viruses
Fundamental and applied biological sciences. Psychology
In Situ Hybridization - veterinary
Invertebrate aquaculture
Larva - virology
Marine
Scylla serrata
White spot syndrome virus
Asia
Taiwan
ISEW, Taiwan
Tropism
Benthic organism
Farming animal
Scylla serrata
Brachyura
Experimental study
Host virus relation
Crustacea
Infection
Marine environment
Polymerase chain reaction
Tissue
Larva
Arthropoda
Viral disease
Decapoda
Invertebrata
Detection
Aquaculture
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Summary:White spot syndrome virus (WSSV), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. In this study, benthic larvae of the mud crab Scylla serrata were captured from Taiwan's coastal waters and screened for the presence of WSSV by polymerase chain reaction (PCR) and in situ hybridization. WSSV was detected in around 60% of the larvae, and this prevalence rate remained fairly constant when the captured larvae were subsequently maintained in an aerated system in the laboratory. WSSV-free larvae obtained from a hatchery were challenged by immersion in a WSSV inoculum. Fifteen days after challenge, cumulative mortality in the experimental group reached 43% compared to 20% in the control group. PCR detection of WSSV in both moribund and surviving specimens clearly implicated the virus as the cause of death in most cases. Histological and in situ hybridization data confirmed that WSSV tissue tropism in Scylla serrata crab larvae is similar to that found in shrimp.
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ISSN:0177-5103
1616-1580
DOI:10.3354/dao040157