Real-Time DNA Sequencing in the Antarctic Dry Valleys Using the Oxford Nanopore Sequencer

The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from environmental microbiology to viral epidemics. Here, we demonstrate the application of offline DNA sequencing of environmental samples using...

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Bibliographic Details
Published inJournal of biomolecular techniques Vol. 28; no. 1; pp. 2 - 7
Main Authors Johnson, Sarah S, Zaikova, Elena, Goerlitz, David S, Bai, Yu, Tighe, Scott W
Format Journal Article
LanguageEnglish
Published United States Association of Biomolecular Resource Facilities 01.04.2017
Subjects
Antarctic Regions
Bacteriophage lambda - genetics
Desert Climate
Environmental Microbiology
High-Throughput Nucleotide Sequencing - instrumentation
Molecular Typing - instrumentation
Reference Standards
Sequence Analysis, DNA - instrumentation
Short Communication
MinION
Antarctica
Nanopore sequencing
extremophiles
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Summary:The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from environmental microbiology to viral epidemics. Here, we demonstrate the application of offline DNA sequencing of environmental samples using a hand-held nanopore sequencer in a remote field location: the McMurdo Dry Valleys, Antarctica. Sequencing was performed using a MK1B MinION sequencer from Oxford Nanopore Technologies (ONT; Oxford, United Kingdom) that was equipped with software to operate without internet connectivity. One-direction (1D) genomic libraries were prepared using portable field techniques on DNA isolated from desiccated microbial mats. By adequately insulating the sequencer and laptop, it was possible to run the sequencing protocol for up to 2½ h under arduous conditions.
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ISSN:1524-0215
1943-4731
DOI:10.7171/jbt.17-2801-009