Visualizing real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a Positron Emitting Tracer Imaging System (PETIS)

• Published in: Journal of experimental botany Vol. 50; no. 334; pp. 637 - 643
• Main Authors: Nakanishi, Hiromi, Bughio, Naimatullah, Matsuhashi, Shinpei, Ishioka, Noriko-Shigeta, Uchida, Hiroshi, Tsuji, Atsunori, Osa, Akihiko, Sekine, Toshiaki, Kume, Tamikazu, Mori, Satoshi
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.05.1999; OXFORD UNIVERSITY PRESS; Oxford Publishing Limited (England)
• Subjects: [11C]methionine; Absorption. Translocation of ions and substances. Permeability; Agronomy. Soil science and plant productions; Amino acids; Barley; Biological and medical sciences; Biosynthesis; discrimination centre; Economic plant physiology; Fe deficiency; Fundamental and applied biological sciences. Psychology; Imaging; Leaves; mugineic acid; Nutrition. Photosynthesis. Respiration. Metabolism; PETIS; Plant physiology and development; Plant roots; Plants; Positrons; Radioactive decay; Regulation of Growth, Development and Whole Organism Physiology; Tillers; Water and solutes. Absorption, translocation and permeability; Visualization; Monocotyledones; Hordeum vulgare; Biological transport; Positron emitter; Methionine; Carbon Isotopes; Functional deficit; Iron; Radioactive tracers; Real time; Cereal crop; Phytosiderophore; Siderophore; Gramineae; Imaging; Angiospermae; Chelating agent; Spermatophyta; Intraspecific comparison
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/50.334.637

[11C]methionine was supplied to Fe-deficient and Fe-sufficient barley plants through a single leaf, and real time 11C movement was monitored using a Positron Emitting Tracer Imaging System (PETIS). In Fe-deficient plants, [11C]methionine was translocated from the tip of the absorbing leaf to the ‘discrimination centre’ located at the base of the shoot, and then retranslocated to all the chlorotic leaves within 60 min, while a negligible amount was retranslocated to the roots. In Fe-sufficient plants, methionine was translocated to the discrimination centre and then only to the newest leaf on the main shoot within 60 min. A negligible amount was also retranslocated to the roots. In conclusion, methionine from the above-ground parts of a plant is not a precursor of mugineic acid under Fe-deficiency. The discrimination centre is suggested to play a vital role in the distribution of mineral elements and metabolites in graminaceous monocots.