miR156/SPL10 Modulates Lateral Root Development, Branching and Leaf Morphology in Arabidopsis by Silencing AGAMOUS-LIKE 79

The developmental functions of miR156-SPL regulatory network have been extensively studied in Arabidopsis, but the downstream genes regulated by each SPL have not been well characterized. In this study, Next Generation Sequencing-based transcriptome analysis was performed on roots of wild type (WT)...

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Published inFrontiers in plant science Vol. 8; p. 2226
Main Authors Gao, Ruimin, Wang, Ying, Gruber, Margaret Y, Hannoufa, Abdelali
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 04.01.2018
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Summary:The developmental functions of miR156-SPL regulatory network have been extensively studied in Arabidopsis, but the downstream genes regulated by each SPL have not been well characterized. In this study, Next Generation Sequencing-based transcriptome analysis was performed on roots of wild type (WT) and miR156 overexpression (miR156OE) plants. One of the genes, , which represses lateral root growth in Arabidopsis, was significantly downregulated in miR156OE plants. A transcription factor, ( ), was also significantly downregulated in the miR156OE plants, but was upregulated in the overexpression (SPL10OE) Arabidopsis plants. In addition, SPL10 was found to bind to the core consensus SPL binding sequences in gene. Moreover, analyses of complementation lines revealed a linear relationship between and in regulating Arabidopsis plant development. In addition, it was observed that plant phenotypes are AGL79 dose-dependent, with higher expression causing narrow leaf shape, less number of leaves and early flowering time, whereas relatively lower AGL79 overexpression produce plants with more rosette leaves and more lateral branches. Our findings revealed direct binding of SPL10 to promoter, which further suggests a role for miR156/SPL10 module in plant lateral root growth by directly regulating .
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Edited by: Jin-Gui Chen, Oak Ridge National Laboratory (DOE), United States
This article was submitted to Plant Cell Biology, a section of the journal Frontiers in Plant Science
Reviewed by: Gang Wu, Zhejiang A & F University, China; Haiyang Wang, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, China
Present Address: Ying Wang, Department of Biology, Carleton University, Ottawa, ON, Canada
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.02226