Isolation of csm1 encoding a class V chitin synthase with a myosin motor-like domain from the rice blast fungus, Pyricularia oryzae

• Published in: FEMS microbiology letters Vol. 170; no. 1; pp. 131 - 139
• Main Authors: Park, I.C, Horiuchi, H, Hwang, C.W, Yeh, W.H, Ohta, A, Ryu, J.C, Takagi, M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 1999; Blackwell; Oxford University Press
• Subjects: Amino Acid Sequence; Amino acids; Ascomycota - enzymology; Ascomycota - genetics; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Southern; C-Terminus; Chitin; Chitin synthase; Chitin Synthase - chemistry; Chitin Synthase - genetics; Chitin Synthase - metabolism; Conserved sequence; DNA, Fungal - chemistry; DNA, Fungal - isolation & purification; Fundamental and applied biological sciences. Psychology; fungal diseases of plants; Fungal plant pathogens; Fungi; genbank/ab018251; Genes, Fungal; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Homology; Introns; Magnaporthe grisea; Microbiology; Molecular Sequence Data; Mycelia; Mycology; Myosin; Myosin motor‐like domain; Myosins - chemistry; Myosins - genetics; Myosins - metabolism; Oryza - microbiology; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; plant health; plant pathogenic fungi; Polymerase Chain Reaction - methods; Pyricularia oryzae; Restriction Mapping; Rice blast; Rice blast fungus; Sequence Analysis, DNA; Variation, races, biotypes, parasitic specialization, genetics; Myosin motor-like domain; Rice blast fungus; Chitin synthase; Pyricularia grisea; Monocotyledones; Plant pathogen; Nucleotide sequence; Enzyme; Transferases; Glycosyltransferases; Peptide chain; Subunit; Cereal crop; Fungi; Characterization; Oryza sativa; Gramineae; Angiospermae; Myosin; Restriction map; Hexosyltransferases; Spermatophyta; Fungi Imperfecti; Aminoacid sequence; Thallophyta; Structural analysis
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1999.tb13365.x

A gene encoding a chitin synthase with a myosin motor-like domain (csm1) was isolated from Pyricularia oryzae using a PCR fragment amplified from a fungal chitin synthase conserved region. The deduced amino acid sequence of csm1 is homologous to that of CsmA of Aspergillus nidulans (65% identity). The putative gene product of csm1 is consisted of the myosin motor-like domain and a chitin synthase domain as in A. nidulans csmA. The chitin synthase domain of its C-terminus was also homologous to Aspergillus fumigatus ChsE (61.4% identity) and Ustilago maydis Chs6 (48.6% identity) that encode class V chitin synthases. Northern analysis demonstrated that the csm1 was expressed throughout the mycelial growth of P. oryzae. This is the first report on the isolation of the gene encoding a class V chitin synthase with the myosin motor-like domain from P. Oryzae.