Assays for investigating deSUMOylation enzymes

Post-translational modifications by the SUMO (Small Ubiquitin-like MOdifier) family of proteins are recently discovered essential regulatory mechanisms. All SUMO proteins are synthesized as larger precursors that are matured by SUMO-specific proteases, known as SENPs, which remove several C-terminal...

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Bibliographic Details
Published inCurrent protocols in molecular biology (Print) Vol. Chapter 10; p. Unit10.30
Main Authors Madu, Ikenna G, Chen, Yuan
Format Journal Article
LanguageEnglish
Published United States 01.07.2012
Subjects
Animals
Endopeptidases - analysis
Endopeptidases - genetics
Endopeptidases - isolation & purification
Endopeptidases - metabolism
Enzyme Assays - methods
Fluorescence Resonance Energy Transfer - methods
Humans
Proteins - metabolism
Small Ubiquitin-Related Modifier Proteins - metabolism
Sumoylation
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Summary:Post-translational modifications by the SUMO (Small Ubiquitin-like MOdifier) family of proteins are recently discovered essential regulatory mechanisms. All SUMO proteins are synthesized as larger precursors that are matured by SUMO-specific proteases, known as SENPs, which remove several C-terminal amino acids of SUMO to expose the Gly-Gly motif. SENPs also remove SUMO modifications from target proteins, making this modification highly dynamic. At least six deSUMOylation enzymes, all of which are encoded by essential genes, have been identified in mammals. SENP1 has been shown to play an important role in the development of prostate cancer and in angiogenesis. This unit describes and discusses methods for characterizing the deSUMOylation enzymes. These assays enable the identification of inhibitors of these enzymes and investigation of their mechanism of inhibition in order to develop research tools and future therapeutics.
ISSN:1934-3647
DOI:10.1002/0471142727.mb1030s99