Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides

• Published in: The plant pathology journal Vol. 27; no. 1; pp. 37 - 43
• Main Authors: Lian, Sen, Seoul National University, Seoul, Republic of Korea, Jonson, Miranda Gilda, Seoul National University, Seoul, Republic of Korea, Cho, W.K., Seoul National University, Seoul, Republic of Korea, Choi, H.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Je, Y.H., Seoul National University, Seoul, Republic of Korea, Kim, K.H., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.03.2011
• Subjects: antibody; ARROZ; NCP; RICE; rice stripe virus; RIZ; 농학; NCP; antibody; rice; rice stripe virus
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2011.27.1.037

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.