Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome

Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vit...

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Bibliographic Details
Published inJournal of helminthology Vol. 93; no. 5; pp. 539 - 547
Main Authors Ambrosio, J.R., Palacios-Arreola, M.I., Ríos-Valencia, D.G., Reynoso-Ducoing, O., Nava-Castro, K.E., Ostoa-Saloma, P., Morales-Montor, J.
Format Journal Article
LanguageEnglish
Published Cambridge, UK Cambridge University Press 01.09.2019
Subjects
Actin
Androgens
Bioinformatics
Biological activity
Cell death
Cysticercosis
Cytoskeleton
Dihydrotestosterone
Ethanol
Hormones
Infections
Isoforms
Laboratory animals
Metabolism
Molecular weight
Molecular weight distribution
Parasites
Pattern analysis
Physiology
Progesterone
Proteins
Receptors
Research Paper
Sex hormones
Taenia crassiceps
Testosterone
Transduction
Tubulin
Worms
United States > US
Mexico
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Summary:Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X18000706