Characteristics of Cucumber mosaic virus Infecting Zucchini in Korea

• Published in: The plant pathology journal Vol. 26; no. 2; pp. 139 - 148
• Main Authors: Kim, M.K., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kwak, H.R., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Jeong, S.G., Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, Republic of Korea, Ko, S.J., Jeonnam Agricultural Research and Extension Services, Kurye, Republic of Korea, Lee, S.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, K.H., Seoul National University, Seoul, Republic of Korea, Choi, J.K., Kangwon National University, Chuncheon, Republic of Korea, Choi, H.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Cha, B.J., Chungbuk National University, Cheongju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.06.2010
• Subjects: CALABACIN; complete genome sequence; COURGETTE; CUCUMBER MOSAIC CUCUMOVIRUS; CUCUMOVIRUS DEL MOSAICO DEL PEPINO; CUCUMOVIRUS MOSAIQUE DU CONCOMBRE; MARROWS; phylogenetic analysis; 농학; phylogenetic analysis; zucchini; CMV; complete genome sequence
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2010.26.2.139

A virus causing stunt, yellowing, severe mosaic, malformation symptoms on leaves and uneven development and malformation on fruits of zucchini was prevalent around Goseong, Gyeongsangnam-do, Korea. A survey conducted (2004) in the Goseong area revealed about 20% virus infection rate. The disease causative identified as Cucumber mosaic virus (CMV-Z1) was further characterized. The isolate induces mosaic symptoms on Cucumis sativus, while severe mosaic, stunt and malformation on C. pepo. Thin section analyses have shown that virus inclusions are formed in the cuticle layers as well as epidermal, parenchyma and collenchymas cells in virus-infected Nicotiana tabacum. CMV-Z1 isolate induced specific cytoplasmic inclusion bodies such as irregular clumps (IC), crystal (Cr) and irregular chloroplasts (ICh). IC was made up of virus particles interspersed with a darkly stained amorphous material and found both in the cytoplasm and vacuoles, whereas ICh and Cr were rarely found in the vacuoles. The genome of CMV-Z1 RNA-1 consists of 3359 nucleotide (nt) encoding la protein of 993 amino acids (aa). The CMV-Z1 RNA-2 was 3050 nt in length containing 2a (857 aa) and 2b (110 aa), while RNA-3 encoding 3a movement protein (279 aa) and coat protein (218 aa) was 2215 nt in length. Phylogenetic analyses of nucleotide sequences of CMV-Zl isolate appeared it is more closely related to subgroup IA than to subgroup IB or II.