Role of Langerhans cells, Ki-67 protein and apoptosis in acquired cholesteatoma: prospective clinical study

• Published in: Journal of laryngology and otology Vol. 127; no. 3; pp. 252 - 259
• Main Authors: Akdogan, V, Yilmaz, I, Canpolat, T, Ozluoglu, L N
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.03.2013
• Subjects: Adolescent; Adult; Apoptosis; Biomarkers, Tumor - metabolism; Cell Proliferation; Child; Cholesteatoma, Middle Ear - metabolism; Cholesteatoma, Middle Ear - pathology; Cholesteatoma, Middle Ear - surgery; Chronic Disease; Female; Hearing loss; Humans; Inflammation; Keratin; Ki-67 Antigen - analysis; Ki-67 Antigen - metabolism; Langerhans Cells - metabolism; Langerhans Cells - pathology; Main Articles; Male; Middle Aged; Otitis Media - diagnosis; Otitis Media - surgery; Otolaryngology; Pathogenesis; Pathology; Patients; Prospective Studies; Proteins; Staining and Labeling; Surgery; Tomography; Young Adult; Cell Proliferation; Cholesteatoma; Langerhans Cells; Apoptosis
• ISSN: 0022-2151; 1748-5460
• DOI: 10.1017/S0022215112003180

To investigate the role of Langerhans cells in the pathogenesis and clinical picture of middle-ear cholesteatoma. The study included 40 patients operated upon for a diagnosis of chronic otitis due to acquired cholesteatoma. A closed surgical technique was used in 20 per cent of patients and an open technique in 80 per cent. Langerhans cells were more densely accumulated in cholesteatoma epithelium, compared with external ear canal skin (p < 0.001). Staining for Ki-67 protein was greater in cholesteatoma epithelium (p < 0.001) and Apo2.7 protein staining (indicating apoptosis) was more prominent (p < 0.001), compared with ear canal skin. Regarding significant relationships between clinical and pathological findings, staining for Ki-67 (p = 0.046) and Apo2.7 (p = 0.037) was more prominent in patients undergoing open versus closed surgery. Using cell proliferation and apoptosis markers, a dense Langerhans cell infiltration was found to occur as a host response to middle-ear cholesteatoma.