Adsorption of α-amylase on dextrin immobilized on kieselguhr or chitin

Dextrin prepared from corn starch was immobilized on kieselguhr or chitin using a crosslinking reagent (epichlorohydrin), to produce a new adsorbent having a high affinity for α-amylase. The dextrins were immobilized at levels of 300–340 and 200–270 mg/g adsorbent on chitin and kieselguhr, respectiv...

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Bibliographic Details
Published inCarbohydrate polymers Vol. 34; no. 1; pp. 55 - 59
Main Authors Kurakake, M., Ueki, M., Hashimoto, S., Komaki, T.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 05.12.1997
Elsevier Science
Subjects
Applied sciences
Exact sciences and technology
Natural polymers
Physicochemistry of polymers
Starch and polysaccharides
Enzyme
Liquid solid adsorption
Epichlorhydrin
Adsorption capacity
Dextrin
Immobilization
Crosslinking
Chitin
Experimental study
Property processing relationship
Glycosidases
Preparation
Crosslinked polymer
Reaction time
Hydrolases
Oside polymer
α-Amylase
Adsorbent
O-Glycosidases
Kieselguhr
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Summary:Dextrin prepared from corn starch was immobilized on kieselguhr or chitin using a crosslinking reagent (epichlorohydrin), to produce a new adsorbent having a high affinity for α-amylase. The dextrins were immobilized at levels of 300–340 and 200–270 mg/g adsorbent on chitin and kieselguhr, respectively. There was an optimum crosslinking time for α-amylase adsorption because of a balance between the number of adsorption sites and the affinity of the immobilized dextrin for α-amylase. Adsorption of α-amylase on the crosslinked immobilized dextrin was as much as 50 times higher than the value on raw corn starch. When expressed in terms of the content the adsorption of α-amylase varied with the type of support (kieselguhr and chitin) because of the different state of the immobilized dextrin.
ISSN:0144-8617
1879-1344
DOI:10.1016/S0144-8617(97)00098-2