Comparison of Interferon Action in Interferon Resistant and Sensitive L1210 Cells

• Published in: Journal of general virology Vol. 41; no. 1; pp. 161 - 166
• Main Authors: Vandenbussche, P, Content, J, Lebleu, B, Werenne, J
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.10.1978
• Subjects: Animals; Cell-Free System; Interferons - pharmacology; Leucine; Leukemia L1210; Mice; Phosphoproteins - metabolism; Phosphorylation; Protein Biosynthesis - drug effects; RNA, Transfer, Amino Acyl - metabolism; Vesicular stomatitis Indiana virus - growth & development
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-41-1-161

Instituut Pasteur van Brabant, B-1040 Brussels, Belgium Département de Biologie Moléculaire, U.L.B., B-1640 Rhode St Genése, Belgium J. Wérenne International Institute of Cellular and Molecular Pathology, B-1200 Brussels, Belgium Translation inhibition, leu-tRNA aminoacylation and double-stranded RNA and ATP dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse L 1210 R and L 1210 S cells. No differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67K dalton phosphoprotein fraction in interferon-treated L 1210 S cell-free extracts. In non-responding cell-free lysates, the lack of stimulation of a 67K dalton phosphoprotein fraction cannot be explained by the presence of an increased level of some inhibitory activity, such as a phosphatase. * Present address: U.S.T.L., Service de Biochimie des Protéines, Place E. Bataillon, 34060 Montpellier, France. Received 23 February 1978; accepted 5 May 1978.