Mixed Infection of 16S rDNA Ⅰ and Ⅴ Groups of Phytoplasma in a Single Jujube Tree

• Published in: The plant pathology journal Vol. 25; no. 1; pp. 21 - 25
• Main Authors: Lee, S.H. (Chungbuk National University, Cheongju, Republic of Korea), Han, S.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: sshan@chonbuk.ac.kr, Cha, B.J. (Chungbuk National University, Cheongju, Republic of Korea)
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.03.2009
• Subjects: 16S rDNA; FITOPLASMAS; jujube witches broom; mulberry dwarf phytoplasma; PHYTOPLASMAS; PHYTOPLASME; RFLP; 농학; phytoplasma; 16S rDNA; jujube witches broom; mulberry dwarf phytoplasma; RFLP
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2009.25.1.021

Jujube trees infected with phytoplasma exhibit symptoms of typical witches' broom, such as yellowing, abnormally small leaves, short internodes and proliferation of shoots. A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches' broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA Ⅰ and Ⅴ groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu Ⅰ, Hha Ⅰ, Msp Ⅰ, and Rsa Ⅰ clearly classified the two into different phytoplasma groups. The phylogenic analyses based on 16S rDNA showed that the similarity of the two different clones was 87.5%. This is the first report of a mixed phytoplasmal infection in a single jujube tree.