Colitis-induced changes in the level of trace elements in rat colon and other tissues

• Published in: Annals of nutrition and metabolism Vol. 42; no. 5; pp. 304 - 310
• Main Authors: Al-Awadi, F.M, Khan, I, Dashti, H.M, Srikumar, T.S
• Format: Journal Article
• Language: English
• Published: Switzerland 1998
• Subjects: Acetic Acid; animal models; Animals; blood serum; colitis; Colitis - chemically induced; Colitis - metabolism; colon; Colon - metabolism; copper; Copper - blood; Copper - metabolism; enzyme activity; glutathione peroxidase; Glutathione Peroxidase - metabolism; liver; Liver - metabolism; Male; manganese; Manganese - blood; Manganese - metabolism; mineral content; peroxidase; Peroxidase - metabolism; Rats; Rats, Sprague-Dawley; selenium; Selenium - blood; Selenium - metabolism; Trace Elements - blood; Trace Elements - metabolism; Trinitrobenzenesulfonic Acid; zinc; Zinc - blood; Zinc - metabolism
• ISSN: 0250-6807; 1421-9697
• DOI: 10.1159/000012748

Trace elements constitute important prosthetic groups in a number of antioxidant enzymes which neutralize free radicals generated during inflammatory conditions such as colitis. However, the status of trace elements in colitis remains to be found. In the present study the concentrations of zinc, copper, manganese and selenium in the colon, liver and serum of rats with acetic acid (HAc)- or trinitrobenzenesulfonic acid (TNBS)-induced colitis were measured using atomic absorption spectrophotometer. Mycloperoxidase and glutathione peroxidase activities were measured spectrophotometrically. Our results show that the selenium concentration was significantly decreased by 33 and 37.5% in the colon and 69 and 78% in liver by HAc and TNBS treatment, respectively. Similarly the zinc concentration in the colon was decreased by 21 and 28% by HAc- and TNBS-induced colitis as compared to the controls, but manganese and copper, remained unaltered. The serum concentrations of copper, zinc and selenium also remained unaltered during colitis. The weight of HAc-treated rats did not decrease while there was a significant weight loss in the TNBS-treated rats. Myeloperoxidase activity was increased, whereas glutathione peroxidase activity was significantly decreased in the colon inflamed by HAc or TNBS as compared to the controls. These findings suggest that colitis induces a reduction in the tissue levels of trace elements which is independent of the way colitis is induced. Our findings of a reduction in Se and glutathione peroxidase activity together suggest that the reduction in the trace element concentrations is not due to dietary factors or malabsorption. The decrease may severely affect the antioxidant potential of the colon and therefore is a putative factor for the progression of disease.