First Report of Cucumber mosaic virus Infecting Pinewood Coneflower (Rudbeckia bicolor) in Korea

• Published in: The plant pathology journal Vol. 26; no. 1; pp. 93 - 98
• Main Authors: Kim, M.K., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kwak, H.R., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Ko, S.J., Jeonnam Agricultural Research and Extension Service, Kurye, Republic of Korea, Lee, S.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Kim, K.H., Seoul National University, Seoul, Republic of Korea, Cha, B.J., Chungbuk National University, Cheongju, Republic of Korea, Choi, H.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.03.2010
• Subjects: CUCUMBER MOSAIC CUCUMOVIRUS; CUCUMOVIRUS DEL MOSAICO DEL PEPINO; CUCUMOVIRUS MOSAIQUE DU CONCOMBRE; phylogenetic analyses; Rudbeckia bicolor; 농학; CMV-Rb; subgroup; Rudbeckia bicolor; phylogenetic analyses
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2010.26.1.093

A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.