High-performance liquid chromatographic method with column-switching and post-column reaction for determination of serotonin levels in platelet-poor plasma

Objectives: Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma...

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Published inClinical biochemistry Vol. 37; no. 3; pp. 191 - 197
Main Authors Hirowatari, Yuji, Hara, Katsuko, Kamihata, Hiroshi, Iwasaka, Toshiji, Takahashi, Hakuo
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.2004
Subjects
Analysis of Variance
Blood Platelets - chemistry
Blood Platelets - metabolism
Chromatography
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Female
Humans
Hydroxyindoleacetic Acid - blood
Male
Middle Aged
Platelet-poor plasma
Serotonin
Serotonin - blood
Platelet-poor plasma
PPP
SD
CV
Serotonin
5-HIAA
EDTA2K
EDTA
EDTA2Na
Chromatography
HPLC
P/WB
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Summary:Objectives: Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma (PPP). Methods: Venous blood samples were collected using vacuum tubes containing ethylenediaminetetraacetic acid (EDTA) dipotassium salt; the optimum concentration in blood was 7.4 mmol/l. Serotonin in samples was separated by HPLC with a column-switching system, and was specifically converted into a fluorescent derivative with benzylamine for convenient detection. Results: The between-day assay coefficient of variation for a serotonin level (5.1 nmol/l) in PPP was 6.2%. The mean concentration of serotonin in PPP in healthy subjects was 5.7 ± 3.0 nmol/l. The serotonin levels in PPP in patients with ischemic heart disease were significantly higher than those in healthy subjects ( P = 0.001). Conclusions: The developed method had sufficient performance to determine serotonin levels in PPP from patients with ischemic heart disease.
Bibliography:ObjectType-Article-2
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ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2003.11.009