Oxidative stress-induced modifications of histidyl-tRNA synthetase affect its tRNA aminoacylation activity but not its immunoreactivity

• Published in: Biochemistry and cell biology Vol. 89; no. 6; pp. 545 - 553
• Main Authors: van Dooren, Sander H J, Raijmakers, Reinout, Pluk, Helma, Lokate, Angelique M C, Koemans, Tom S, Spanjers, Richelle E C, Heck, Albert J R, Boelens, Wilbert C, van Venrooij, Walther J, Pruijn, Ger J M
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.12.2011; Canadian Science Publishing NRC Research Press
• Subjects: Amino Acid Sequence; Antibody Specificity; Apoptosis; Autoantibodies - blood; Autoantibodies - metabolism; Catalysis; Dermatomyositis - blood; Dermatomyositis - immunology; Enzyme Activation; Enzymes; Histidine-tRNA Ligase - metabolism; histidyl-ARNt synthétase; histidyl-tRNA synthetase; Humans; Hydrogen Peroxide - pharmacology; Immunoblotting; Jo-1; Jurkat Cells; Methionine - metabolism; modification post-traductionnelle; Molecular Sequence Data; Musculoskeletal diseases; myosite; myositis; Oxidative Stress; Polymyositis - blood; Polymyositis - immunology; posttranslational modification; Ribonucleic acid; RNA; stress oxydant; Tandem Mass Spectrometry; Transfer RNA Aminoacylation; Tryptophan - metabolism
• ISSN: 0829-8211; 1208-6002
• DOI: 10.1139/o11-055

The aminoacyl-tRNA synthetases are ubiquitously expressed enzymes that catalyze the esterification of amino acids to their cognate tRNAs. Autoantibodies against several aminoacyl-tRNA synthetases are found in autoimmune polymyositis and dermatomyositis patients. Because necrosis is often found in skeletal muscle biopsies of these patients, we hypothesized that cell-death-induced protein modifications may help in breaking immunological tolerance. Since cell death is associated with oxidative stress, the effect of oxidative stress on the main myositis-specific autoantibody target Jo-1 (histidyl-tRNA synthetase; HisRS) was studied in detail. The exposure of Jurkat cells to hydrogen peroxide resulted in the detection of several oxidized methionines and one oxidized tryptophan residue in the HisRS protein, as demonstrated by mass spectrometry. Unexpectedly, the tRNA aminoacylation activity of HisRS appeared to be increased upon oxidative modification. The analysis of myositis patient sera did not lead to the detection of autoantibodies that are specifically reactive with the modified HisRS protein. The results of this study demonstrate that the Jo-1/HisRS autoantigen is modified under oxidative stress conditions. The consequences of these modifications for the function of HisRS and its autoantigenicity are discussed.