An Unexpected Behavior of Horseradish Peroxidase in AOT Reversed Microemulsion:UV spectroscopic and Stopped-Flow Studies

Intermediate compound II and III may be formed as a result of the reaction of horseradish peroxidase with H 2 O 2 . From the UV spectra, we can see that the stability of the intermediate compounds is worse in reverse micelle than that in aqueous solution. With the increase of the water content, the...

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Bibliographic Details
Published inJournal of dispersion science and technology Vol. 20; no. 1-2; pp. 425 - 436
Main Authors Ma, Chengsong, Li, Ganzuo, Shen, Runnan, Li, Shuben, Wang, Hangqing
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Taylor & Francis Group 01.01.1999
Taylor & Francis
Subjects
Chemistry
Colloidal state and disperse state
Emulsions. Microemulsions. Foams
Exact sciences and technology
General and physical chemistry
Anionic surfactant
Stability
Peroxidases
Enzyme
Oxidoreductases
Aqueous solution
Experimental study
Reverse micelle
Microemulsion
Ultraviolet spectrometry
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Summary:Intermediate compound II and III may be formed as a result of the reaction of horseradish peroxidase with H 2 O 2 . From the UV spectra, we can see that the stability of the intermediate compounds is worse in reverse micelle than that in aqueous solution. With the increase of the water content, the stability of intermediate compounds gets better, this means that the characteristic of the water in the water pool is near that of bulk water. The results of the stopped-flow experiments show that the formation rate constant and V initial of intermediate compound in reverse microemulsion are bigger than that in aqueous solution. However, within die longer reaction time, the disappearance of the intermediate compound appears early in reverse microemulsion than that in aqueous solution. These results are in agreement with the result of the UV experiments.
ISSN:0193-2691
1532-2351
DOI:10.1080/01932699908943800