Algorithms for mapping kidney tissue oxygenation during normothermic machine perfusion using hyperspectral imaging

The lack of donor grafts is a severe problem in transplantation medicine. Hence, the improved preservation of existing and the usage of organs that were deemed untransplantable is as urgent as ever. The development of novel preservation techniques has come into focus. A promising alternative to trad...

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Published inBiomedizinische Technik Vol. 63; no. 5; pp. 557 - 566
Main Authors Markgraf, Wenke, Feistel, Philipp, Thiele, Christine, Malberg, Hagen
Format Journal Article
LanguageEnglish
German
Published Germany De Gruyter 25.10.2018
Walter de Gruyter GmbH
Subjects
Algorithms
Calibration
Cold storage
Cryopreservation
discrete wavelength method
Evaluation
Humans
Hyperspectral imaging
Kidney - pathology
Kidneys
Magnetic Resonance Spectroscopy
Organs
Oxygen
Oxygen content
oxygen saturation
Oxygenation
partial least square regression
Perfusion
Perfusion - instrumentation
Perfusion - methods
Regression analysis
tissue parameters
Tissues
Transplantation
Wavelength
tissue parameters
oxygen saturation
partial least square regression
hyperspectral imaging
discrete wavelength method
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Summary:The lack of donor grafts is a severe problem in transplantation medicine. Hence, the improved preservation of existing and the usage of organs that were deemed untransplantable is as urgent as ever. The development of novel preservation techniques has come into focus. A promising alternative to traditional cold storage is normothermic machine perfusion (NMP), which provides the benefit of improving the organs’ viability and of assessing the organs’ status under physiological conditions. For this purpose, methods for evaluating organ parameters have yet to be developed. In a previous study, we determined the tissue oxygen saturation (StO ) of kidneys during NMP with hyperspectral imaging (HSI) based on a discrete wavelength (DW) algorithm. The aim of the current study was to identify a more accurate algorithm for StO calculation. A literature search revealed three candidates to test: a DW algorithm and two full spectral algorithms – area under a curve and partial least square regression (PLSR). After obtaining suitable calibration data to train each algorithm, they were evaluated during NMP. The wavelength range from 590 to 800 nm was found to be appropriate for analyzing StO of kidneys during NMP. The PLSR method shows good results in analyzing the tissues’ oxygen status in perfusion experiments.
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ISSN:0013-5585
1862-278X
DOI:10.1515/bmt-2017-0216