True one cell chemical analysis: a review
The constantly growing field of True One Cell (TOC) analysis has provided important information on the direct chemical composition of various cells and cellular components. Since the heterogeneity of individual cells has been established, more researchers are interested in the chemical differences b...
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| Published in | Analyst (London) Vol. 144; no. 16; pp. 4733 - 4749 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Royal Society of Chemistry
05.08.2019
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| Subjects | |
| Online Access | Get full text |
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| Summary: | The constantly growing field of True One Cell (TOC) analysis has provided important information on the direct chemical composition of various cells and cellular components. Since the heterogeneity of individual cells has been established, more researchers are interested in the chemical differences between individual cells; TOC is the only form of analysis that can provide this information. This has resulted in the constant development of new technologies and methods. This review highlights the common techniques for micro- and nanomanipulation, Raman spectroscopy, microscopy, and mass spectrometric imaging as they pertain to TOC chemical analysis.
The constantly growing field of True One Cell (TOC) analysis has provided important information on the direct chemical composition of various cells and cellular components. |
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| Bibliography: | Holly-May Lewis received her Chemistry BSc Hons degree from the University of Surrey. She is currently a PhD student under the supervision of Dr Melanie Bailey and Professor Roger Webb at the Advanced Technology Institute, University of Surrey. Her current research is focused on spatially resolved liquid chromatography mass spectrometry of biological samples. Guido Verbeck graduated from the Department of Chemistry at Texas A&M University in 2004. Guido Verbeck is a Professor of Chemistry and Biochemistry at the University of North Texas and Director of the Laboratory of Imaging Mass Spectrometry. His research interest focuses on instrument development for Mass Spectrometry (MS), specifically in Preparative MS, field portable MS, and nano-extraction for ultra-trace analysis. He has been a member of the Analytical Community since 1996. Imesha W. De Silva received her BS degree in chemistry from The Open University of Sri Lanka (OUSL) in December 2013. Currently, she is a Graduate Student in the Laboratory of Imaging Mass Spectrometry under Dr. Guido Verbeck while she pursues a PhD in analytical chemistry at the University of North Texas. Her current research is focused on nanomanipulation coupled to mass spectrometry in determination of oxidative stress-related chemical biomarkers in the True One Cell (TOC) analysis. Melanie Bailey is a Senior Lecturer in Analytical and Forensic Science at the University of Surrey. She has recently been awarded a five-year fellowship from the Electronics and Physical Sciences Research Council, which is exploring spatially resolved mass spectrometry and ion beam analysis (for high resolution elemental mapping). Amanda Kretsch received her BS in chemistry from the University of North Texas in 2014. She attended graduate school at the University of North Texas and received her MS in analytical chemistry under Dr. Guido Verbeck in 2018. Her thesis research covers the analysis of harmful chemicals in the air using portable mass spectrometry. She currently works as an analytical scientist at a toxicology lab. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
| ISSN: | 0003-2654 1364-5528 |
| DOI: | 10.1039/c9an00558g |