nucleic acid hydrolyzing recombinant antibody confers resistance to curtovirus infection in tobacco

A catalytic single chain variable antibody (scFv), 3D8 scFv, which has DNase activities, was functionally expressed in Nicotiana tabacum. The subcellular localization of the GFP-fused 3D8 indicated that the 3D8 protein was expressed in the cytosol of the N. tabacum protoplasts. Progenies of the tran...

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Published inPlant cell, tissue and organ culture Vol. 115; no. 2; pp. 179 - 187
Main Authors Lee, Gunsup, Shim, Hye-Kyung, Kwon, Myung-Hee, Son, Su-Hwan, Kim, Ki-Yoon, Park, Eul-Yong, Lee, Taek-Kyun, Lee, Woo-Ram, Auh, Chung-Kyoon, Kim, Donggiun, Kim, Yong-Sung, Lee, Sukchan
Format Journal Article
LanguageEnglish
Published Dordrecht Springer-Verlag 01.11.2013
Springer Netherlands
Springer Nature B.V
Subjects
adverse effects
Antibodies
Beet curly top virus
Beet severe curly top virus
Biomedical and Life Sciences
Catalysis
Curtovirus
Cytosol
Deoxyribonuclease
Deoxyribonucleic acid
DNA
Life Sciences
Localization
Nicotiana tabacum
Nucleotide sequence
nucleotide sequences
Original Paper
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant protection
Plant Sciences
Plant viruses
Proteins
Protoplasts
reproduction
Signs and symptoms
single-stranded DNA
Tobacco
Transgenic plants
viral proteins
Viruses
Geminivirus
3D8 scFv
DNase
Antibody
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Summary:A catalytic single chain variable antibody (scFv), 3D8 scFv, which has DNase activities, was functionally expressed in Nicotiana tabacum. The subcellular localization of the GFP-fused 3D8 indicated that the 3D8 protein was expressed in the cytosol of the N. tabacum protoplasts. Progenies of the transgenic tobacco plants exhibited complete resistance against two single stranded (ss) DNA geminiviruses, including the Beet curly top virus and the Beet severe curly top virus, without viral accumulation or disease symptoms. We presented a novel strategy for targeting the viral DNA itself in a sequence non-specific manner, rather than the viral proteins or RNAs, in order to generate virus-resistant transgenic plants. No noticeable adverse effects on the growth and reproduction of the transgenic plants were observed. Our results demonstrated that targeting viral DNA is an effective strategy for protecting plants from ssDNA viruses.
Bibliography:http://dx.doi.org/10.1007/s11240-013-0357-4
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-013-0357-4