Cardiac glycosides in partly submerged shoots of Digitalis lanata

Shoot cultures were established from axillary buds (11 strains) or seeds (1 strain) of individual Digitalis lanata Ehrh. plants and propagated partially submerged in liquid medium. Five of these shoot culture strains were characterized with regard to their growth and cardenolide content. The culture...

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Published inPlanta medica Vol. 59; no. 6; p. 539
Main Authors Stuhlemmer, U. (Tuebingen Univ. (Germany). Pharmazeutisches Institut), Kreis, W, Eisenbeiss, M, Reinhard, E
Format Journal Article
LanguageEnglish
Published Germany 01.12.1993
Subjects
BIOSINTESIS
BIOSYNTHESE
cardenolides
Cardenolides - analysis
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CULTIVO DE MERISTEMAS
CULTURE DE MERISTEME
Culture Techniques
Darkness
DIGITALIS
Digitalis - chemistry
Digitalis Glycosides - analysis
Digitalis lanata
GLICOSIDOS
GLYCOSIDE
Light
PLANTAS MEDICINALES
PLANTE MEDICINALE
Plants, Medicinal
Plants, Toxic
Scrophulariaceae
shoot culture
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Summary:Shoot cultures were established from axillary buds (11 strains) or seeds (1 strain) of individual Digitalis lanata Ehrh. plants and propagated partially submerged in liquid medium. Five of these shoot culture strains were characterized with regard to their growth and cardenolide content. The cultures were observed for more than one year and found to be relatively stable with regard to their growth and cardenolide spectrum and yield. The strains examined differed in terms of their total cardenolide yield, which ranged from about 30nmol g per DW to almost 1000nmol g per DW. Cardenolide content was correlated with leaf size and development. Depending on the strain investigated up to ten different cardenolides could be detected by HPLC. The main cardenolides were identified by comparing HPLC and TLC results with those of authentic samples and chemical degradation as being the mono- and diglycosides glucodigifucoside, glucoverodoxin, odorobioside G, and odoroside H; minor amounts of digitalinum verum and glucoevatromonoside were also found. In addition, the tetrasaccharides lanatoside A and C were present. The shoots were cardenolide-free when cultivated in the dark for more than 30 weeks, but regained their characteristic cardenolide profile when transferred back to light. For the dark cultivation of chlorophyll-free cultures a medium containing 3.5 % glucose was found to be optimal.
Bibliography:F60
94B0058
ISSN:0032-0943
1439-0221
DOI:10.1055/s-2006-959757