Aptamer-functionalized AlGaN/GaN High-electron-mobility Transistor for Rapid Diagnosis of Fibrinogen in Human Plasma

• Published in: Sensors and materials Vol. 30; no. 10; p. 2321
• Main Authors: Tai, Tse-Yu, Sinha, Anirban, Sarangadharan, Indu, Pulikkathodi, Anil Kumar, Wang, Shin-Li, Shiesh, Shu-Chu, Lee, Gwo-Bin, Wang, Yu-Lin
• Format: Journal Article
• Language: English
• Published: Tokyo MYU Scientific Publishing Division 01.01.2018
• Subjects: Aluminum gallium nitrides; Biosensors; Blood plasma; Debye length; Diagnosis; Diagnostic systems; Fibrinogen; Heart diseases; Selectivity; Sensitivity; Transistors
• ISSN: 0914-4935
• DOI: 10.18494/SAM.2018.1938

In this research, we have developed a rapid diagnostic assay using a high-electron-mobility transistor (HEMT)-based biosensor to screen for fibrinogen in clinical plasma samples. High sensitivity over a wide detection range (0–5 g/L), good selectivity, low-cost fabrication, portability, and simplicity of use are key features of our biosensor. Early diagnosis of heart disease in low-, medium-, and high-risk patients is possible with an assay turn-around time of 5 min and a plasma sample volume of 5–10 µL. High sensitivity and low detection limits (0.5 g/L) are made possible in environments with high salt concentrations because the HEMT sensor design overcomes the charge screening effect associated with a very small Debye length. This biosensor technology demonstrates the potential for development as a point-of-care or home-care diagnostic device for quick and easy assessment of cardiovascular disease (CVD) risk.