Identification of TyeA residues required to interact with YopN and to regulate Yop secretion

• Published in: Advances in experimental medicine and biology Vol. 603; p. 235
• Main Authors: Joseph, Sabrina S, Plano, Gregory V
• Format: Journal Article
• Language: English
• Published: United States 2007
• Subjects: Alanine - chemistry; Amino Acid Substitution; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - physiology; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - physiology; Base Sequence; Binding Sites - genetics; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - physiology; DNA, Bacterial - genetics; Genes, Bacterial; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane Proteins - physiology; Models, Molecular; Mutagenesis, Site-Directed; Mutation, Missense; Phenotype; Point Mutation; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Yersinia pestis - genetics; Yersinia pestis - pathogenicity; Yersinia pestis - physiology
• ISSN: 0065-2598
• DOI: 10.1007/978-0-387-72124-8_21

The secretion of Yops via the Yersinia type III secretion system (T3SS) is controlled, in part, by a cytoplasmic YopN/TyeA complex. This complex is required to prevent Yop secretion in the presence of extracellular calcium and prior to contact between the bacterium and a eukaryotic cell. In this study we utilized site-directed mutagenesis to analyze the role of specific TyeA regions and residues in the regulation of Yop secretion. We identified two spatially distinct, surface-exposed regions of the TyeA molecule that were required to regulate Yop secretion. One region, identified by residues M51, F55 and P56, was required for TyeA to interact with YopN. A second region, identified by residues R19, W20 and D25 was not involved in the interaction of TyeA with YopN, but may be required for the YopN/TyeA complex to interact with the T3S apparatus in a manner that blocks Yop secretion.