Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall

• Published in: Plant cell, tissue and organ culture Vol. 92; no. 3; pp. 251 - 260
• Main Authors: Xue, Sheng-Hui, Luo, Xin-Juan, Wu, Zhen-Hua, Zhang, Hui-Li, Wang, Xin-Yu
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.03.2008; Springer Netherlands; Springer
• Subjects: Astragalus; Astragalus membranaceus; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; cold storage; cryopreservation; encapsulation-vitrification method; Eruca sativa; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; genetic transformation; Gentiana; Gentiana macrophylla; hairy roots; Life Sciences; medicinal plants; Methods. Procedures. Technologies; Miscellaneous; Original Paper; Plant cells and fungal cells; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Rhizobium rhizogenes; roots; transgenic plants; Encapsulation–vitrification; Mill; Hairy root; Cold storage; Pall; Cryopreservation; Encapsulation; Cold; Vitrification; Eruca sativa Mill; Eruca sativa; Encapsulation-vitrification; Medicinal plant; Storage; Cruciferae; Dicotyledones; Angiospermae; Gentiana macrophylla Pall; Spermatophyta; Culture; Astragalus membranaceus
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-007-9329-x

To explore the possibility of an effectively long-term preservation of the germplasm of the HR lines of medicinal plant Astragalus membranaceus, Gentiana macrophylla Pall., and Eruca sativa Mill., both cold storage and cryopreservation approaches were attempted and compared. After 5-month cold storage on half strength Murashige and Skoog (1962) (1/2 MS) agar medium (AM), up to 82.9, 75.7, and 100% of the A. membranaceus, G. macrophylla and E. sativa hairy roots (HRs) recovered growth, respectively. The survival rates of A. membranaceus and G. macrophylla HRs significantly decreased, whereas that of E. sativa HR was unchanged with the addition of increased levels of exogenous abscisic acid (ABA) during cold storage. Using the encapsulation-vitrification (EV) method for cryopreservation, the G. macrophylla HRs died, whereas up to 6 and 73% of the A. membranaceus and E. sativa HRs survived, respectively. The HR lines evaluated with both methods showed no significant differences in morphology and growth rate compared with controls that were not subjected to preservation methods. These results suggest that cold storage is a more suitable alternative for the HR lines of the three studied plant species and that specificity of plant species have profound effects on the effectiveness of preservation.