Analysis of Major Components of Bacitracin, Colistin and Virginiamycin in Feed Using Matrix Solid-phase Dispersion Extraction by Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry

• Published in: Journal of chromatographic science Vol. 56; no. 3; pp. 285 - 291
• Main Authors: Tao, Yanfei, Xie, Shuyu, Zhu, Yufei, Chen, Dongmei, Pan, Yuanhu, Wang, Xu, Liu, Zhenli, Huang, Lingli, Peng, Dapeng, Yuan, Zonghui
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.03.2018
• Subjects: Animal Feed - analysis; Bacitracin - analysis; Bacitracin - chemistry; Bacitracin - isolation & purification; Chromatography, Liquid - methods; Colistin - analysis; Colistin - chemistry; Colistin - isolation & purification; Drug Residues - analysis; Drug Residues - chemistry; Drug Residues - isolation & purification; Limit of Detection; Linear Models; Reproducibility of Results; Solid Phase Extraction - methods; Spectrometry, Mass, Electrospray Ionization - methods; Virginiamycin - analysis; Virginiamycin - chemistry; Virginiamycin - isolation & purification
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/bmx096

Abstract A quantitative LC-MS/MS method has been developed for simultaneous determination of bacitracin A, bacitracin B, colistin A, colistin B and virginiamycin M1 in feed. This rapid simple and effective extraction method was based on matrix solid-phase dispersion. Qualitative and quantitative analyses were performed by LC-ESI-MS/MS. CCβ of polypeptide antibiotics upon the method ranged from 9.6 to 15.8 μg kg−1 and 19.4 to 27.5 μg kg−1, respectively. The limit of quantification of polypeptide antibiotics was 25 μg kg−1 in feed samples. The recoveries of polypeptide antibiotics spiked in feed samples at a concentration range of 25-100 μg kg−1 were found above 75.9-87.9% with relative standard deviations within days less than 15.7% and between days less than 20.6%. This rapid and reliable method can be used to efficiently separate, characterize and quantify the residues of polypeptide antibiotics in feed with advantages of simple pretreatment and environmental friendly.