Determination of [13C-15N] Labeled Methylxanthines by Gas Chromatography-Atomic Emission Detection (GC-AED) and Compound-Independent Calibration

Compound independent calibration (CIC) can be used to measure stable isotopically labeled molecules and their metabolites. This study was designed to demonstrate that gas chromatography coupled to atomic emission detection and CIC are useful analytical tools for the specific and quantitative determi...

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Bibliographic Details
Published inAnalytical letters Vol. 32; no. 15; pp. 3111 - 3130
Main Authors Elbast, W., Caubet, M.S., Deruaz, D., Brazier, J.L.
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Taylor & Francis Group 01.01.1999
Taylor & Francis
Subjects
Analysis
Atomic Emission Detection
Biological and medical sciences
Compound-Independent Calibration - CIC
General pharmacology
Medical sciences
N] Labeled methylxanthines- Stable isotopes
Pharmacology. Drug treatments
Chemical analysis
Nitrogen 15
Nitrogen heterocycle
Metabolite
Coupled method
Gas chromatography
Emission spectrometry
Multicomponent analysis
Bicyclic compound
Xanthine derivatives
Pharmacokinetics
Carbon 13
Labelled compound
Quantitative analysis
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Summary:Compound independent calibration (CIC) can be used to measure stable isotopically labeled molecules and their metabolites. This study was designed to demonstrate that gas chromatography coupled to atomic emission detection and CIC are useful analytical tools for the specific and quantitative determination of 13 C and 15 N labeled caffeine and its metabolites. These labeled compounds can be measured by monitoring either 13 C or 15 N atomic emission lines and using only one calibration function from one of the analytes. This method can be helpful when it is difficult or impossible to obtain an authentic reference material, which is often the case in metabolic studies.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719908543030