Effect of Germinated Soybean Protein Hydrolysates on Adipogenesis and Adipolysis in 3T3-L1 Cells

• Published in: Plant foods for human nutrition (Dordrecht) Vol. 66; no. 4; pp. 355 - 362
• Main Authors: González-Espinosa de los Monteros, L. A., Ramón-Gallegos, E., Torres-Torres, N., Mora-Escobedo, R.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.11.2011; Springer
• Subjects: 3T3-L1 Cells; Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis - drug effects; Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts; Animals; Biological and medical sciences; Cell Survival - drug effects; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Ecology; Feeding. Feeding behavior; Food industries; Food Science; Fundamental and applied biological sciences. Psychology; Germination; Glycine max - chemistry; Glycine max - metabolism; Lipolysis - drug effects; Mice; Nutrition; Original Paper; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Physiology; Protein Hydrolysates - isolation & purification; Protein Hydrolysates - metabolism; Protein Hydrolysates - pharmacology; Soybean Proteins - isolation & purification; Soybean Proteins - metabolism; Soybean Proteins - pharmacology; Time Factors; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Adipolysis; Soybean; Germination; 3T3-L1; Hydrolysates; Adipogenesis; Adipocyte; Evaluation; Vegetables; Extract; Bioavailability; Bean; Soy protein; Grain legume; Hydrolysate; Nutrient; Concentrate; Day; Lipolytic
• ISSN: 0921-9668; 1573-9104
• DOI: 10.1007/s11130-011-0263-z

Germination of soybeans increases the bioavailability of some nutrients. An evaluation was done to determine if germination increased the anti-adipogenic and lipolytic effects of soybean. Soybeans were germinated for 0 to 6 days and protein concentrates extracted from beans germinated at each period. Soy protein concentrates can retain notable amounts of phytochemicals with anti-adipogenic activity. For this reason, it was evaluated the effect of protein hydrolysates with and without phytochemicals in the adipocyte-like cells after 3T3-L1 (murine fibroblasts) cell line differentiation. Cell viability decreased with exposure to the germinated soybean protein hydrolysates during the differentiation stage, but not during the fibroblast or mature adipocyte stages. Adipogenesis and triglycerides accumulation were strongly inhibited by the hydrolysate from soybeans germinated for 2 days (with ethanol-soluble phytochemicals), when compared to ungerminated soybean. Adipolysis increased with exposure to hydrolysates from beans germinated for 2 days (with phytochemicals) and 5 days (without phytochemicals). Germinated soy protein hydrolysates had an effect on inhibition of lipid storage in adypocites and increasing lipolysis, which was improved by changes of the protein and increased phytochemical content due to germination.