Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow

A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, pr...

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Published inOrganic process research & development Vol. 24; no. 10; pp. 2281 - 2287
Main Authors Poznansky, Barnabas, Thompson, Lisa A., Warren, Sarah A., Reeve, Holly A., Vincent, Kylie A.
Format Journal Article
LanguageEnglish
Published American Chemical Society 16.10.2020
Subjects
Communication
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Summary:A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5–11 and space-time yields of up to 22.9 g L –1 h –1 . The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.
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ISSN:1083-6160
1520-586X
DOI:10.1021/acs.oprd.9b00410