Molecular Prediction of Rat Liver Triglycerides by High Performance Liquid Chromatography

• Published in: Journal of liquid chromatography & related technologies Vol. 21; no. 8; pp. 1185 - 1198
• Main Authors: Perona, J. S., Barrón, L. J. R., Ruiz-Gutiérrez, V.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.04.1998; Taylor & Francis
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Glycerolipids, phospholipids; Lipids; Other biological molecules; Elution; Gradient; Chemical analysis; Rat; Chromatographic retention; Light scattering; Liver; Rodentia; Prediction; HPLC chromatography; Lipids; Regression analysis; Triglyceride; Fatty acids; Vertebrata; Reversed phase chromatography; Mammalia; Analysis method; Triacylglycerol; Correlation analysis; Animal; Chemical composition; Property structure relationship; Detection
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826079808006593

Triglyceride composition of rat liver was analyzed by HPLC with a non-linear elution gradient of acetone in acetonitrile using a light-scattering detector. Triglyceride molecular species were predicted by means of simple and multiple linear regression analysis between the log k' of the HPLC peaks and molecular variables, equivalent carbon number (ECN) of the possible triglycerides, and chain length (CL) and number of double bounds (DB) of each of the fatty acids in the glycerol molecule. The triglycerides presenting high random percentages were considered the principal molecular species in each HPLC peak. The main molecular species of rat liver triglycerides were palmitoyl-oleoyl-linoleoyl-glycerol, triolein, palmitoyl-dilinoleoyl-glycerol, dipalmitoyl-oleoyl-glycerol and oleoyl-dilinoleoyl-glycerol. The systematics predicted molecular species with arachidonic acid, such as dioleoyl-arachidonoyl-glycerol, dipalmitoyl-arachidonoyl-glycerol, and oleoyl-linoleoyl-arachidonoyl-glycerol, and with docosahexaenoic acid, including dipalmitoyl-docosahexaenoyl-glycerol, palmitoyl-oleoyl-docosa-hexaenoyl-glycerol, and palmitoyl-linoleoyl-docosahexaenoyl-glycerol.