Transformation of 2- and 4-cyanopyridines by free and immobilized cells of nitrile-hydrolyzing bacteria

• Published in: Applied biochemistry and microbiology Vol. 49; no. 4; pp. 347 - 351
• Main Authors: Maksimova, Yu. G, Vasilyev, D. M, Ovechkina, G. V, Maksimov, A. Yu, Demakov, V. A
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.07.2013; Springer US; Springer Nature B.V
• Subjects: amidase; Amides; Bacteria; Biochemistry; Biomedical and Life Sciences; Carboxylic acids; Cells; coal; immobilized cells; Inorganic chemistry; kaolin; Life Sciences; Medical Microbiology; Microbiology; nitrilase; Pseudomonas; Pseudomonas fluorescens; Rhodococcus; Rhodococcus ruber; Picolinic Acid; Isonicotinic Acid; Nitrile Hydratase; Apply Biochemistry; Immobilize Cell
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S000368381304008X

The transformation dynamics of 2- and 4-cyanopyridines by cells suspended and adsorbed on inorganic carriers has been studied in the Rhodococcus ruber gt1 possessing nitrile hydratase activity and the Pseudomonas fluorescens C2 containing nitrilase. It was shown that both nitrile hydratase and nitrilase activities of immobilized cells against 2-cyanopyridine were 1.5–4 times lower compared to 4-cyanopyridine and 1.6–2 times lower than the activities of free cells against 2-cyanpopyridine. The possibility of obtaining isonicotinic acid during the combined conversion of 4-cyanopyridine by a mixed suspension of R. ruber gt1 cells with a high level of nitrile hydratase activity and R. erythropolis 11-2 cells with a pronounced activity of amidase has been shown. Immobilization of Rhodococcus cells on raw coal and Pseudomonas cells on kaolin was shown to yield a heterogeneous biocatalyst for the efficient transformation of cyanopyridines into respective amides and carboxylic acids.