Intrauterine growth restriction and placental gene expression in severe preeclampsia, comparing early-onset and late-onset forms

• Published in: Journal of perinatal medicine Vol. 45; no. 7; pp. 869 - 877
• Main Authors: Nevalainen, Jaana, Skarp, Sini, Savolainen, Eeva-Riitta, Ryynänen, Markku, Järvenpää, Jouko
• Format: Journal Article
• Language: English
• Published: Germany De Gruyter 26.10.2017; Walter de Gruyter GmbH
• Subjects: Adult; Adverse pregnancy outcome; Case-Control Studies; Female; Fetal Growth Retardation - metabolism; Gene expression; Gene Expression Profiling; Health risk assessment; Humans; intrauterine growth restriction; microarray; Placenta - metabolism; Pre-Eclampsia - metabolism; Preeclampsia; Pregnancy; Prenatal development; severe early-onset preeclampsia; severe late-onset preeclampsia; Young Adult; Adverse pregnancy outcome; intrauterine growth restriction; severe early-onset preeclampsia; microarray; severe late-onset preeclampsia
• ISSN: 0300-5577; 1619-3997
• DOI: 10.1515/jpm-2016-0406

To evaluate placental gene expression in severe early- or late-onset preeclampsia with intrauterine growth restriction compared to controls. Chorionic villus sampling was conducted after cesarean section from the placentas of five women with early- or late-onset severe preeclampsia and five controls for each preeclampsia group. Microarray analysis was performed to identify gene expression differences between the groups. Pathway analysis showed over-representation of gene ontology (GO) biological process terms related to inflammatory and immune response pathways, platelet development, vascular development, female pregnancy and reproduction in early-onset preeclampsia. Pathways related to immunity, complement and coagulation cascade were overrepresented in the hypergeometric test for the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Ten genes (ABI3BP, C7, HLA-G, IL2RB, KRBOX1, LRRC15, METTL7B, MPP5, RFLNB and SLC20A) had a ≥±1 fold expression difference in severe early-onset preeclampsia group compared to early controls. There were 362 genes that had a ≥±1 fold expression difference in severe early-onset preeclampsia group compared to late-onset preeclampsia group including ABI3BP, C7, HLA-G and IL2RB. There are significant differences in placental gene expression between severe early- and late-onset preeclampsia when both are associated with intrauterine growth restriction. ABI3BP, C7, HLA-G and IL2RB might contribute to the development of early form of severe preeclampsia.