QTL analysis of canning quality and color retention in black beans (Phaseolus vulgaris L.)

• Published in: Molecular breeding Vol. 33; no. 1; pp. 139 - 154
• Main Authors: Cichy, Karen A, Fernandez, Andrea, Kilian, Andrzej, Kelly, James D, Galeano, Carlos H, Shaw, Scott, Brick, Mark, Hodkinson, Donny, Troxtell, Emily
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 2014; Springer Netherlands; Springer Nature B.V
• Subjects: anthocyanins; ASP gene; Beans; Biomedical and Life Sciences; Biotechnology; black beans; Canning; canning industry; canning quality; chromosome mapping; Chromosomes; Color; Cultivars; Gene mapping; genes; Genomes; inbred lines; Inbreeding; Life Sciences; Luster; Marker-assisted selection; Markers; Molecular biology; Nucleotide sequence; nucleotide sequences; Phaseolus vulgaris; phenotypic variation; Phenotypic variations; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Quality assessment; Quantitative trait loci; Retention; Seed coats; Technology assessment; testa; Water quality; Water uptake; United States; Linkage map; Anthocyanins; Consumer acceptance; Processing quality; gene
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-013-9940-y

Black bean (Phaseolus vulgaris L.) consumption is increasing in the USA. One of the major challenges faced by breeders is to develop superior black bean cultivars to meet the demands of the canning industry. Processors require beans that take up water quickly during pre-canning soak and beans that retain their black color after canning. To properly assess canning quality requires expensive and detailed measurements of the canned product, often not possible for bean breeders. The objective of this research was identify quantitative trait loci (QTL) in a black bean recombinant inbred line (RIL) population for canning quality traits related to water uptake, color retention, and anthocyanin concentration. The parental lines from which the population was developed, Black Magic and Shiny Crow, contrasted in water uptake and color retention. These cultivars also differed in seed coat luster, controlled by a single gene, Asp. A medium-density linkage map of 1,449 markers and a distance of 1,660 cM was developed from this RIL population. The map was aligned to the bean genome sequence V1.0 by using sequence information associated with the Diversity Arrays Technology markers. QTL analysis revealed that the region near the Asp gene on chromosome Pv 07 is the major determinant of water uptake, explaining up to 49 % of the phenotypic variation. A group of QTL for color retention-related traits was found at the upper region of Pv 11, explaining up to 30 % of the phenotypic variation. A smaller effect QTL clustered on Pv 5 co-localized with a QTL for canned bean anthocyanin concentration and explained less than 10 % of the phenotypic variation. These color-related QTL have marker-assisted selection potential for bean breeders interested in enhancing color retention and anthocyanin concentration of processed black beans.