Selective recognition of CG interruption by 2′,4′-BNA having 1-isoquinolone as a nucleobase in a pyrimidine motif triplex formation
To develop a novel nucleoside analogue for the effective recognition of CG interruption in a homopurine–homopyrimidine tract of double-stranded DNA (dsDNA), we succeeded in the synthesis of a triplex-forming oligonucleotide (TFO) containing a novel 2′,4′-BNA (Q B) bearing 1-isoquinolone as a nucleob...
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Published in | Tetrahedron Vol. 59; no. 27; pp. 5123 - 5128 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
30.06.2003
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Subjects | |
Online Access | Get full text |
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Summary: | To develop a novel nucleoside analogue for the effective recognition of CG interruption in a homopurine–homopyrimidine tract of double-stranded DNA (dsDNA), we succeeded in the synthesis of a triplex-forming oligonucleotide (TFO) containing a novel 2′,4′-BNA (Q
B) bearing 1-isoquinolone as a nucleobase, and the triplex-forming ability and sequence-selectivity of the TFO (TFO-Q
B) were examined. On melting temperature (
T
m) measurements, it was found that the TFO-Q
B formed a stable triplex DNA in a highly sequence-selective manner under near physiological conditions.
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ISSN: | 0040-4020 1464-5416 |
DOI: | 10.1016/S0040-4020(03)00728-2 |