Selective recognition of CG interruption by 2′,4′-BNA having 1-isoquinolone as a nucleobase in a pyrimidine motif triplex formation

To develop a novel nucleoside analogue for the effective recognition of CG interruption in a homopurine–homopyrimidine tract of double-stranded DNA (dsDNA), we succeeded in the synthesis of a triplex-forming oligonucleotide (TFO) containing a novel 2′,4′-BNA (Q B) bearing 1-isoquinolone as a nucleob...

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Bibliographic Details
Published inTetrahedron Vol. 59; no. 27; pp. 5123 - 5128
Main Authors Hari, Yoshiyuki, Obika, Satoshi, Sekiguchi, Mitsuaki, Imanishi, Takeshi
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 30.06.2003
Subjects
antigene
molecular recognition
nucleic acid analogues
triplex
molecular recognition
triplex
nucleic acid analogues
antigene
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Summary:To develop a novel nucleoside analogue for the effective recognition of CG interruption in a homopurine–homopyrimidine tract of double-stranded DNA (dsDNA), we succeeded in the synthesis of a triplex-forming oligonucleotide (TFO) containing a novel 2′,4′-BNA (Q B) bearing 1-isoquinolone as a nucleobase, and the triplex-forming ability and sequence-selectivity of the TFO (TFO-Q B) were examined. On melting temperature ( T m) measurements, it was found that the TFO-Q B formed a stable triplex DNA in a highly sequence-selective manner under near physiological conditions. Graphic
ISSN:0040-4020
1464-5416
DOI:10.1016/S0040-4020(03)00728-2