Acid and alkaline ceramidases of rat tissues

Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-14C]oleoyl- and N-[9,10-3H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissues and tissue areas examined, and b...

Full description

Saved in:
Bibliographic Details
Published inBiochemistry and cell biology Vol. 64; no. 5; p. 400
Main Authors Spence, M W, Beed, S, Cook, H W
Format Journal Article
LanguageEnglish
Published Canada 01.05.1986
Subjects
Amidohydrolases - metabolism
Animals
Brain - enzymology
Carbon Radioisotopes
Ceramidases
Drug Stability
Female
Hot Temperature
Hydrogen-Ion Concentration
Kidney - enzymology
Kinetics
Male
Rats
Tissue Distribution
Online AccessGet more information

Cover

More Information
Summary:Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-14C]oleoyl- and N-[9,10-3H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissues and tissue areas examined, and both activities decreased in the following order: kidney greater than brain stem greater than cerebrum greater than cerebellum greater than liver greater than spleen greater than cardiac muscle greater than lung greater than psoas muscle. The distribution of activity is not as tissue specific as that of neutral Mg2+-stimulated sphingomyelinase which is enriched in cells of neural origin and thus suggests a less specialized role for the alkaline ceramidase.
ISSN:0829-8211
DOI:10.1139/o86-056