Protease Activity on the Surface of HSV-infected Cells

• Published in: Journal of general virology Vol. 45; no. 1; pp. 241 - 244
• Main Authors: Dierich, M. P, Landen, B, Schulz, Th, Falke, D
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.10.1979
• Subjects: Animals; Cell Membrane - enzymology; Cells, Cultured; Complement Activation; Complement C3 - metabolism; Kidney; Peptide Hydrolases - metabolism; Phenylmethylsulfonyl Fluoride - pharmacology; Rabbits; Simplexvirus - growth & development; Tosyllysine Chloromethyl Ketone - pharmacology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-45-1-241

Institute for Medical Microbiology and Division of Experimental Virology University of Mainz 6500 Mainz, West Germany Monolayers of primary rabbit kidney cells infected with HSV type 1 bound lymphoblastoid (Raji) cells, to which the third component (C3) of the complement system had been attached (Raji-C3). This induction of cell contact did not occur on non-infected monolayers and was dependent on C3. The interaction could be suppressed by the presence of protease inhibitors (1 m M -TLCK or-PMSF). The results are interpreted as indicating de novo expression of protease activity on the surface of HSV-infected cells. This protease is characterized by its potential to activate C3 and its inhibition by TLCK. Received 19 January 1979; accepted 4 June 1979.