Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation

• Published in: Applied biochemistry and microbiology Vol. 49; no. 6; pp. 581 - 586
• Main Authors: Osmolovsky, A. A, Kreier, V. G, Baranova, N. A, Kurakov, A. V, Egorov, N. S
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.11.2013; Springer US; Springer Nature B.V
• Subjects: Aspergillus alutaceus; Aspergillus ochraceus; Biochemistry; Biomedical and Life Sciences; blood plasma; culture media; Fermentation; Fungi; Life Sciences; Medical Microbiology; Microbiology; Micromycetes; Plasma; Proteases; proteinases; solid state fermentation; Fermentation; Wheat Bran; Activate Activity; Apply Biochemistry; Solid State Fermentation
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683813060148

The conditions for the submerged and solid-state fermentation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C of human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state culture was 1.5-3.5 times higher than in a submerged culture (as calculated per milliliter of culture medium). Among the extracellular proteins secreted by A. ochraceus VKM F-4104D during submerged and solid-state fermentation, a protein C-activating proteinase with a pI of 6.0–6.3 was identified.