Preparation of a multifunctional photoactivated prodrug on a streptavidin scaffold bearing a DNA aptamer

[Display omitted] Prodrugs that present strong cytotoxicity toward specific cells have been utilized for cell-type selection and purification. In this study, we designed and prepared a multifunctional, photoactivated prodrug based on a streptavidin scaffold. Biotin-labeled DNA aptamer that recognize...

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Published inBioorganic & medicinal chemistry letters Vol. 71; p. 128819
Main Authors Motohashi, Yuto, Nishihara, Tatsuya, Tanabe, Kazuhito
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 01.09.2022
Elsevier
Subjects
Chemistry
Chemistry, Medicinal
Chemistry, Organic
Life Sciences & Biomedicine
Pharmacology & Pharmacy
Physical Sciences
Science & Technology
PLURIPOTENT STEM-CELLS
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Summary:[Display omitted] Prodrugs that present strong cytotoxicity toward specific cells have been utilized for cell-type selection and purification. In this study, we designed and prepared a multifunctional, photoactivated prodrug based on a streptavidin scaffold. Biotin-labeled DNA aptamer that recognizes the membrane antigen EpCAM, and biotin-labeled photoactivated prodrug bearing the antitumor camptothecin, were prepared. Both molecules were linked to the streptavidin scaffold by simple mixing. The resulting prodrug bound to the EpCAM-overexpressing SK-BR-3 target cells and showed cytotoxic effects upon photoirradiation, corresponding to cytotoxic drug release.
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ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2022.128819