Immune response to vaccination with DNA encoding the bovine viral diarrhea virus major glycoprotein gp53 (E2)

• Published in: FEMS microbiology letters Vol. 146; no. 2; pp. 229 - 234
• Main Authors: Harpin, Serge, Talbot, Brian, Mbikay, Majambu, Elazhary, Youssef
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1997; Oxford University Press
• Subjects: Animals; Antibodies; Antibodies, Viral - immunology; Biotypes; bovine diarrhea virus; Bovine viral diarrhea virus; Bovine Virus Diarrhea-Mucosal Disease - immunology; Bovine Virus Diarrhea-Mucosal Disease - prevention & control; Cattle; Cloning, Molecular; COS Cells - microbiology; Cytomegalovirus; Deoxyribonucleic acid; Diarrhea; DNA; DNA plasmid immunization; DNA vaccines; DNA, Complementary; DNA, Viral - immunology; DNA, Viral - pharmacology; Female; Gene Expression Regulation, Viral - immunology; Genotype & phenotype; Genotypes; Glycoproteins; gp53 (E2) expression; Immune response; Mice; Mice, Inbred BALB C; Microbiology; Microinjections; Neutralization Tests; Neutralizing; Plasmids; Transfection; Vaccination; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viruses; gp53 (E2) expression; DNA plasmid immunization; Bovine viral diarrhea virus
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1997.tb10198.x

Abstract Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle which has not been controlled by classical vaccination. The region encoding the BVDV major glycoprotein gp53 (E2) known to possess virus-neutralizing activity was cloned into a mammalian expression vector under the human cytomegalovirus (CMV) intermediate early promoter. Intramuscular and intradermal administration of the recombinant plasmid DNA into BALB/c mice induced BVDV gp53-specific antibody responses to both biotypes (cytopathic and noncytopathic) of BVDV genotype 1, and to cytopathic BVDV genotype 2. BVDV-neutralizing antibodies were generated against BVDV genotype 1 strains and they also persisted 6 months after the last injection.