Antifeedant and Antifungal Activities of Metabolites Isolated from the Coculture of Endophytic Fungus Aspergillus tubingensis S1120 with Red Ginseng

• Published in: Chemistry & biodiversity Vol. 19; no. 1; pp. e202100608 - n/a
• Main Authors: Wu, Ya‐Mei, Yang, Xue‐Qiong, Zhao, Tong‐De, Shi, Wen‐Zhi, Sun, Li‐Jingfei, Cen, Rong‐Huan, Yang, Ya‐Bin, Ding, Zhong‐Tao
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01.01.2022
• Subjects: Animals; antifeedant activities; antifungal activities; Antifungal agents; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; Ascomycota - drug effects; Aspergillus - chemistry; Aspergillus - growth & development; Aspergillus - metabolism; Aspergillus tubingensis; Bombyx - drug effects; Bombyx - growth & development; Chemical compounds; Circular dichroism; Dichroism; Disease resistance; Endophytes; Enediynes - chemistry; Enediynes - isolation & purification; Enediynes - pharmacology; Fatty Alcohols - chemistry; Fatty Alcohols - isolation & purification; Fatty Alcohols - pharmacology; Fungicides; Ginseng; Herbal medicine; Insect Repellents - chemistry; Insect Repellents - isolation & purification; Insect Repellents - pharmacology; Medicinal plants; Metabolites; Microbial Sensitivity Tests; Minimum inhibitory concentration; Molecular Conformation; Monoculture; Panax - chemistry; Panax - growth & development; Panax - metabolism; Phoma - drug effects; Plant diseases; Plant immunity; Plants, Medicinal - chemistry; Plants, Medicinal - growth & development; Plants, Medicinal - metabolism; red ginseng; secondary metabolites; Silkworms; Synergistic effect; secondary metabolites; red ginseng; Aspergillus tubingensis; antifungal activities; antifeedant activities
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202100608

A new globoscinic acid derivative, aspertubin A (1) along with four known compounds, were obtained from the co‐culture of Aspergillus tubingensis S1120 with red ginseng. The chemical structures of compounds were characterized by using spectroscopic methods, the calculated and experimental electronic circular dichroism. Panaxytriol (2) from red ginseng, and asperic acid (4) showed significant antifeedant effect with the antifeedant rates of 75 % and 80 % at the concentrations of 50 μg/cm2. Monomeric carviolin (3) and asperazine (5) displayed weak attractant activity on silkworm. All compounds were assayed for antifungal activities against phytopathogens A. tubingensis, Nigrospora oryzae and Phoma herbarum and the results indicated that autotoxic aspertubin A (1) and panaxytriol (2) possessed selective inhibition against A. tubingensis with MIC values at 8 μg/mL. The co‐culture extract showed higher antifeedant and antifungal activities against P. herbarum than those of monoculture of A. tubingensis in ordinary medium. So the medicinal plant and endophyte showed synergistic effect on the plant disease resistance by active compounds from the coculture of A. tubingensis S1120 and red ginseng.