The Role of Rotational Motion in Diffusion NMR Experiments on Supramolecular Assemblies: Application to Sup35NM Fibrils

• Published in: Angewandte Chemie International Edition Vol. 60; no. 28; pp. 15445 - 15451
• Main Authors: Kharkov, Boris B., Podkorytov, Ivan S., Bondarev, Stanislav A., Belousov, Mikhail V., Salikov, Vladislav A., Zhouravleva, Galina A., Skrynnikov, Nikolai R.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 05.07.2021
• Edition: International ed. in English
• Subjects: amyloid fibrils; Amyloidogenesis; Assemblies; Biomolecules; Diffusion; Electron microscopy; Experiments; Fibrils; NMR; NMR spectroscopy; Nuclear magnetic resonance; Prion protein; proteins; Proteolysis; pulsed-field gradient diffusion measurements; Rotational diffusion; Sup35; Sup35; Amyloid fibrils; Pulsed-field gradient diffusion measurements; NMR spectroscopy; proteins
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.202102408

Pulsed‐field gradient (PFG) NMR is an important tool for characterization of biomolecules and supramolecular assemblies. However, for micrometer‐sized objects, such as amyloid fibrils, these experiments become difficult to interpret because in addition to translational diffusion they are also sensitive to rotational diffusion. We have constructed a mathematical theory describing the outcome of PFG NMR experiments on rod‐like fibrils. To test its validity, we have studied the fibrils formed by Sup35NM segment of the prion protein Sup35. The interpretation of the PFG NMR data in this system is fully consistent with the evidence from electron microscopy. Contrary to some previously expressed views, the signals originating from disordered regions in the fibrils can be readily differentiated from the similar signals representing small soluble species (e.g. proteolytic fragments). This paves the way for diffusion‐sorted NMR experiments on complex amyloidogenic samples. Diffusion of amyloid fibrils that contain disordered domains can be captured by pulsed‐field‐gradient NMR experiments. A new theory is presented that interprets the results of these measurements in terms of fibrils’ translational and rotational motion. Application to Sup35NM fibrils demonstrates the feasibility of diffusion‐based sorting in complex amyloidogenic samples.